optogeneticsを用いた、食欲制御に関する論文.
- title: Rapid binge-like eating and body weight gain driven by zona incerta GABA neuron activation
- Authors: Xiaobing Zhang, Anthony N. van den Pol
- Science. 26 May 2017: Vol. 356, Issue 6340, pp. 853-859
- DOI: 10.1126/science.aam7100
使ったツール
- optogenetics
- ChIEF： 興奮タイプのチャネルロドプシン. ChR1とChR2のあいのこ…？
- genetics
- AAV
- vGAT
- RVΔG
AAVdj-CAG-DIO-ChIEF-tdTomato * VGAT-Cre
GABAnegicニューロンでのみChIEFが発現.
- key word
- binge eating
expression pattern: fig. S1
- orexigenic: 食欲亢進
- anorexigenic: 食欲抑制
知らなかった部分、重要な部分
腹側視床の電気刺激が副作用として過食を引き起こす
deep brain stimulation of the subthalamus, including the zona incerta (ZI), for the treatment of movement disorders can exhibit characteristics of binge eating
ZIは未知領域であるが、広い領域への投射が知られてる
The ZI is one of the least-studiedregions of the brain, despite its robust projections throughout the brain
20Hz ZI stim.で一日の消費食事量の35.4%を10分間で食べてしまう
bilateral laser stimulation (20 Hz) in the ZI increased food intake, with mice rapidly consuming 35.4% of their 24-hour ad libitumhigh-fat food intake in just 10 min
10分間のONを4回繰り返すと74%…
10 min ON followed by 30 min OFF was repeated four times,
ZI-VGAT-ChIEF mice consumed 74% of their normal 24-hour food intake, whereas control mice consumed only 22% (Fig. 1E).
従来知られてるAgRPニューロンより刺激後の誘起が爆速だったよ
This is almost 100 times faster than that reported for optogenetic stimulation of the AgRP neuron soma and 500 times faster than stimulation ofAgRP-PVT axon terminals (19, 20).
