読者です 読者をやめる 読者になる 読者になる

たれぱんのびぼーろく

わたしの備忘録、生物学とプログラミングが多いかも

ゼブラフィッシュの核酸がらみ命名規則

transgenic zebrafish

融合配列

ハイフンで繋ぐ

Fusions in constructs: Regulatory or coding sequence fusions should be separated by hyphens.
Example: Tg(actb2:stk11-mCherry)
This construct codes for a fused protein of stk11 and mCherry under the control of the actb2 promoter.

違う長さのプロモーター

マイナス表記
-5xxx == xxx遺伝子CDSの-5kb上流

Promoter elements of differing sizes in constructs: In cases where a number of constructs are generated with different sizes of promoter elements, these may be specified within the parentheses using the length of the upstream DNA: Examples: These examples represent two constructs that code for a fusion protein of sptb and GFP driven by an upstream enhancer either 3.5kb or 6.0kb 5' to the hhex gene. Tg(-3.5hhex:sptb-GFP) Tg(-6.0hhex:sptb-GFP) However, in many cases, the changes within the construct may be too small or too complex to change the number of kbp or cannot be determined. To differentiate these constructs, they will be appended with a sequential number between the Tg (also Et, Pt, Gt) and the parentheses, instead of including further details in the name. Details will be provided in the notes field on the construct page. Examples: original construct: Tg1(uxs1:GFP); subsequent construct: Tg2(uxs1:GFP); additional constructs: Tg#(uxs1:GFP)

lineとconstructは違うよ

line: 系統
construct:

lineは魚.
constructはDNA.